Rapid identification of dermatophyte species by 28S rDNA Polymerase Chain Reaction (PCR)
نویسنده
چکیده
Dermatophytes are the main cause of onychomycoses, but various nondermatophyte filamentous fungi are often isolated from abnormal nails. The correct identification of the aetiological agent of nail infections is necessary in order to recommend appropriate treatment. To evaluate a rapid polymerase chain reaction (PCR) assay based on 28S rDNA for fungal identification in nails on a large number of samples in comparison with cultures. Infectious fungi were analyzed using PCR in 22 samples of swabs and nail samples in which fungal elements were observed in situ by direct mycological examination (positive samples). The results were compared with those previously obtained by culture of fungi on Sabouraud agar from the same samples. PCR identification of fungi in nails allowed validation of the results obtained in culture when three Trichophyton spp. and one Microsporum spp grew from infected samples. Improved sensitivity for the detection of fungi in nails was obtained using the PCR assay. Rapid and reliable molecular identification of the infectious fungus can be used routinely and presents several important advantages compared with culture in expediting the choice of appropriate antifungal therapy.
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